Integrated magnetofluid multicolor immunochip for visual analysis of neutralizing antibodies against SARS-CoV-2 variants

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Anal chemistry. 2022 June 3. doi: 10.1021/acs.analchem.2c01260. Online ahead of print.

ABSTRACT

The global spread of the SARS-CoV-2 virus has seriously affected human health, life and work. Immunization by vaccine is considered an effective way to protect the body against infections. Therefore, rapid antibody level analysis is useful to identify individuals with weak immune response or attenuated antibodies for targeted and accurate booster vaccination. Here, we develop a multicolor, magnetofluid-integrated immunochip, as a sample-response system in a fully enclosed space, for the visual analysis of SARS-CoV-2 neutralizing antibodies. Generally, this chip adopts an innovative three-dimensional two-phase system that uses mineral oil to block the connection between reagent wells in the vertical direction and provides a wide interface for rapid and non-destructive shuttle of magnetic beads during immunoassay. In order to obtain a visualized signal output, gold nanorods with a size-dependent color effect are used as colored chromogenic substrates for antibody level assessment. Thanks to this chip, neutralizing antibodies were successfully detected in vaccinated volunteers with a sensitivity of 83.3% and a specificity of 100%. Additionally, changes in antibody levels of the same individual over time were also reflected by the multi-color test. Overall, benefiting from simple operation, airtight security and non-requirement of external equipment, this platform can provide a novel point-of-care testing strategy to alleviate shortage of medical resources. and promote epidemic control in underdeveloped areas.

PMID:35658117 | DOI:10.1021/acs.analchem.2c01260

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